Functional phosphosite screening for targeted protein-protein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays.
نویسندگان
چکیده
An effective new platform for phosphosite mapping and subsequent functional screening was developed to analyze the targeted protein-protein interactions of p300 and CBP with β-catenin. Two novel functional phosphosites, Ser12 of p300 and Ser92 of CBP, were revealed to modulate p300/β-catenin and CBP/β-catenin interactions, respectively.
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ورودعنوان ژورنال:
- Molecular bioSystems
دوره 7 6 شماره
صفحات -
تاریخ انتشار 2011